Isolation of pathogenic bacteria from sputum samples using a 3D-printed cartridge system-Reference-Cited by-同舟云学术

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Isolation of pathogenic bacteria from sputum samples using a 3D-printed cartridge system

Published:2021 Issue:41 Volume:13 Page:4884-4895
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Pahlow Susanne¹²³^ORCID,Hentschel Stefanie¹²³,Horbert Peter³,Romero Cynthia¹³,Lehniger Lydia¹²³,Wagner Sascha³,Popp Jürgen¹²³^ORCID,Weber Karina¹²³^ORCID

Affiliation:

1. Friedrich Schiller University Jena, Institute of Physical Chemistry and Abbe Center of Photonics, Helmholtzweg 4, 07743 Jena, Germany

2. InfectoGnostics Research Campus Jena, Center for Applied Research, Philosophenweg 7, 07743 Jena, Germany

3. Leibniz Institute of Photonic Technology – Member of the Research Alliance “Leibniz Health Technologies“, Albert-Einstein-Straße 9, 07745 Jena, Germany

Abstract

We introduce a 3D-printed cartridge system enabling the convenient and cost-efficient sample preparation from sputum for PCR based detection schemes.

Funder

Bundesministerium für Bildung und Forschung

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY00924A

Reference18 articles.

1. Inhibition and facilitation of nucleic acid amplification

2. Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification

3. Development of a self-contained sample preparation cartridge for automated PCR testing

4. Fully automated molecular diagnosis by a novel cartridge-based platform

5. Automated Platform for Rapid and Reproducible Sample Preparations in Point-of-Care(POC) Molecular Diagnostics

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