Application of tandem fast protein liquid chromatography to purify intact native monomeric/aggregated Tamm–Horsfall protein from human urine and systematic comparisons with diatomaceous earth adsorption and salt precipitation: yield, purity and time-consumption-Reference-Cited by-同舟云学术

Application of tandem fast protein liquid chromatography to purify intact native monomeric/aggregated Tamm–Horsfall protein from human urine and systematic comparisons with diatomaceous earth adsorption and salt precipitation: yield, purity and time-consumption

Published:2021 Issue:30 Volume:13 Page:3359-3367
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Noonin Chadanat¹²³⁴⁵,Kapincharanon Chompunoot¹²³⁴⁵,Sueksakit Kanyarat¹²³⁴⁵,Kanlaya Rattiyaporn¹²³⁴⁵,Thongboonkerd Visith¹²³⁴⁵^ORCID

Affiliation:

1. Medical Proteomics Unit

2. Office for Research and Development

3. Faculty of Medicine Siriraj Hospital

4. Mahidol University

5. Bangkok 10700

Abstract

The efficiency of tandem FPLC (Mono Q anion-exchange/Superdex 200 size-exclusion) for purification of intact Tamm–Horsfall protein (uromodulin) from human urine was compared with two conventional methods, i.e., salt precipitation and DE adsorption.

Funder

Thailand Research Fund

Faculty of Medicine Siriraj Hospital, Mahidol University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D1AY00922B

Reference26 articles.

1. Uromodulin (Tamm–Horsfall protein): guardian of urinary and systemic homeostasis

2. Uromodulin: from physiology to rare and complex kidney disorders