DNA–EB in agarose gel assay: a simple methodology in the search for DNA-binders in crude extracts from actinomycetes-Reference-Cited by-同舟云学术

DNA–EB in agarose gel assay: a simple methodology in the search for DNA-binders in crude extracts from actinomycetes

Published:2016 Issue:12 Volume:8 Page:2653-2659
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Bauermeister Anelize¹²³⁴⁵,Abdel Baqui Munira Muhammad⁶³⁴⁵,Beraldo Moraes Luiz Alberto¹²³⁴⁵

Affiliation:

1. Mass Spectrometry and Natural Products Laboratory – Departamento de Química da Faculdade de Filosofia

2. Ciências e Letras de Ribeirão Preto

3. Universidade de São Paulo

4. Ribeirão Preto

5. Brazil

6. Departamento de Biologia Celular e Molecular e Bioagentes Patogenicos – Faculdade de Medicina de Ribeirão Preto

Abstract

Here, a methodology was proposed to detect DNA-binders in a screening process with crude extracts produced by actinomycetes, positive extracts were subjected to replication studies by LC-MS/MS.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AY/C5AY02603B

Reference28 articles.

1. DNA as a target for anticancer compounds: methods to determine the mode of binding and the mechanism of action

2. DNA binders in clinical trials and chemotherapy

3. DNA and its associated processes as targets for cancer therapy

4. Role of the amino sugar in the DNA binding of disaccharide anthracyclines: crystal structure of the complex MAR70/d(CGATCG)

5. Study on the Interaction of an Anthracycline Disaccharide with DNA by Spectroscopic Techniques and Molecular Modeling

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