A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification-Reference-Cited by-同舟云学术

A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

Published:2015 Issue:6 Volume:5 Page:4019-4025
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Zhou Fulin¹²³⁴⁵,Li Baoxin¹²³⁴⁵,Ma Jiyuan¹²³⁴⁵

Affiliation:

1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province

2. School of Chemistry & Chemical Engineering

3. Shaanxi Normal University

4. Xi'an 710062

5. China

Abstract

Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/RA/C4RA14467H

Reference32 articles.

1. Ultrasensitive DNA Detection by Cascade Enzymatic Signal Amplification Based on Afu Flap Endonuclease Coupled with Nicking Endonuclease

2. Amplified Analysis of DNA by the Autonomous Assembly of Polymers Consisting of DNAzyme Wires

3. Target-Responsive Structural Switching for Nucleic Acid-Based Sensors

4. DNA-Mediated Homogeneous Binding Assays for Nucleic Acids and Proteins

5. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products

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