Miniaturized single-cell technologies for monoclonal antibody discovery

Author:

Van Lent Julie1ORCID,Breukers Jolien1ORCID,Ven Karen1ORCID,Ampofo Louanne12,Horta Sara3,Pollet Francesca1ORCID,Imbrechts Maya24,Geukens Nick4,Vanhoorelbeke Karen34,Declerck Paul24ORCID,Lammertyn Jeroen1ORCID

Affiliation:

1. Department of Biosystems, Biosensors Group, KU Leuven, Leuven 3001, Belgium

2. Laboratory for Therapeutic and Diagnostic Antibodies, KU Leuven, Leuven 3000, Belgium

3. Laboratory for Thrombosis Research, IRF Life Sciences, KU Leuven Campus Kulak Kortrijk, Kortrijk 8500, Belgium

4. PharmAbs, The KU Leuven Antibody Center, KU Leuven, Leuven 3000, Belgium

Abstract

To discover Ag-specific mAbs using miniaturized single-cell technologies, cells are: (i) confined, (ii) identified, (iii) retrieved, and (iv) sequenced.

Funder

Fonds Wetenschappelijk Onderzoek

H2020 Marie Skłodowska-Curie Actions

KU Leuven

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Reference184 articles.

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2. B.Alberts , A.Johnson , J.Lewis , M.Raff , K.Roberts and P.Walter , B Cells and Antibodies, in Molecular Biology of the Cell 4th edition , 2002

3. Monoclonal Versus Polyclonal Antibodies: Distinguishing Characteristics, Applications, and Information Resources

4. Glycoengineered antibodies: towards the next-generation of immunotherapeutics

5. S. A.Berkowitz , Challenges with the biophysical characterization of complex(multi-chain assembly, chemically modified, big and high concentration) biopharmaceuticals, in Biophysical Characterization of Proteins in Developing Biopharmaceuticals , Elsevier , 2020 , pp. 459–479

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