Isolation of circulating fetal trophoblasts by a four-stage inertial microfluidic device for single-cell analysis and noninvasive prenatal testing-Reference-Cited by-同舟云学术

Isolation of circulating fetal trophoblasts by a four-stage inertial microfluidic device for single-cell analysis and noninvasive prenatal testing

Published:2020 Issue:23 Volume:20 Page:4342-4348
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Huang Yifang¹²³⁴⁵,Yu Sheng¹²³⁴⁵,Chao Shuzhe⁶⁷⁸,Wu Limei⁹¹⁰¹¹⁸,Tao Maliang¹²³⁴⁵,Situ Bo¹²³⁴⁵^ORCID,Ye Xinyi¹²³⁴⁵,Zhang Ye¹²³⁴⁵,Luo Shihua¹²³⁴⁵,Chen Weishan¹²³⁴⁵,Jiang Xiujuan¹²³⁴⁵,Guan Guofeng⁶⁷⁸,Zheng Lei¹²³⁴⁵^ORCID

Affiliation:

1. Department of Laboratory Medicine and

2. Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors

3. Nanfang Hospital

4. Southern Medical University

5. Guangzhou 510515

6. Shenzhen Genflow Technologies Co., Ltd.

7. Shenzhen

8. China

9. Department of Clinical Laboratory

10. Guangzhou Twelfth People's Hospital

11. Guangzhou

Abstract

A novel four-stage inertial microfluidic chip is developed for isolating rare circulating trophoblastic cells from whole blood samples of pregnancies. The antibody-free, low-cost assay may serve as a useful platform for noninvasive prenatal testing.

Funder

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/D0LC00895H

Reference27 articles.

1. Noninvasive prenatal testing for chromosome aneuploidies and subchromosomal microdeletions/microduplications in a cohort of 42,910 single pregnancies with different clinical features

2. Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma

3. Size Distributions of Maternal and Fetal DNA in Maternal Plasma