Structured oligonucleotides for target indexing to allow single-vessel PCR amplification and solid support microarray hybridization-Reference-Cited by-同舟云学术

Structured oligonucleotides for target indexing to allow single-vessel PCR amplification and solid support microarray hybridization

Published:2015 Issue:3 Volume:140 Page:912-921
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Girard Laurie D.¹²³⁴⁵,Boissinot Karel¹²³⁴⁵,Peytavi Régis¹²³⁴⁵,Boissinot Maurice¹²³⁴⁵,Bergeron Michel G.¹²³⁴⁵

Affiliation:

1. Centre de recherche en infectiologie de l'Université Laval

2. Axe maladies infectieuses et immunitaires

3. Centre de recherche du CHU de Québec

4. Québec City

5. Canada

Abstract

A structured oligonucleotide is irreversibly digested in the presence of its complementary target during PCR, releasing a short oligonucleotide tag for microarray hybridization in a single vessel and single reaction mixture.

Funder

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2015/AN/C4AN01352B

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