Spatio-temporally deciphering peripheral nerve regeneration in vivo after extracellular vesicle therapy under NIR-II fluorescence imaging

Author:

Wang Yueming1,Sheng Huaixuan2,Cong Meng3,Wang Wenjin4,He Qianru3,Li Huizhu2ORCID,Li Shunyao2ORCID,Zhang Jian2,Chen Yuzhou5,Guo Shuaicheng67,Fang Lu67,Pluchino Stefano8,Biskup Ewelina910,Artemyev Mikhail11ORCID,Chen Fuchun7,Li Yunxia2,Chen Jun2ORCID,Feng Sijia2ORCID,Wo Yan1

Affiliation:

1. Department of Anatomy and Physiology, School of Medicine, Shanghai Jiao Tong University, Shanghai 200025, China

2. Sports Medicine Institute of Fudan University, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China

3. Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, JS 226001, China

4. Department of Plastic and Reconstructive Surgery. Shanghai ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China

5. Department of Othopedic Surgery, Xin Hua Hospital affiliated to School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China

6. University of Chinese Academy of Sciences, Beijing 100049, China

7. Key Laboratory of Infrared System Detection and Imaging Technology, Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China

8. Department of Clinical Neurosciences, University of Cambridge, Cambridge CB2 0AH, UK

9. Department of Basic and Clinical Science, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China

10. Department of Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China

11. Research Institute for Physical Chemical Problems of the Belarusian State University, Leningradskaya srt., 14, Minsk, 220006, Belarus

Abstract

In this study, a new non-invasive near-infrared fluorescence imaging strategy based on glucose-conjugated quantum dots (QDs-Glu) labeling to target and track EVs in a sciatic nerve injury rat model in real-time was introduced.

Funder

Science and Technology Commission of Shanghai Municipality

Fudan University

National Natural Science Foundation of China

Shanghai Science and Technology Development Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science

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