Microfluidic platform for monitoring Saccharomyces cerevisiae mutation accumulation-Reference-Cited by-同舟云学术

Microfluidic platform for monitoring Saccharomyces cerevisiae mutation accumulation

Published:2021 Issue:12 Volume:21 Page:2407-2416
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Sipos Eliet H.¹²³⁴⁵,Léty-Stefanska Adélaïde¹²³⁶⁷,Denby Wilkes Cyril¹²³⁴⁵,Soutourina Julie¹²³⁴⁵^ORCID,Malloggi Florent¹²³⁶⁷^ORCID

Affiliation:

1. Université Paris-Saclay

2. CEA

3. CNRS

4. Institute for Integrative Biology of the Cell (I2BC)

5. Gif-sur-Yvette

6. NIMBE

7. 91191 Gif-sur-Yvette Cedex

Abstract

We present an innovative microfluidic-based system that automatizes the mutation accumulation culture in budding yeast. We speed up by one order of magnitude the time necessary for genome-wide measurements of mutational profiles.

Funder

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D1LC00086A

Reference31 articles.

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2. Death and population dynamics affect mutation rate estimates and evolvability under stress in bacteria

3. Genomic mutation rates: what high-throughput methods can tell us

4. Genome dynamics during experimental evolution

5. Old Trade, New Tricks: Insights into the Spontaneous Mutation Process from the Partnering of Classical Mutation Accumulation Experiments with High-Throughput Genomic Approaches

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