Synthetic metabolism for <i>in vitro</i> acetone biosynthesis driven by ATP regeneration-Reference-Cited by-同舟云学术

Synthetic metabolism for in vitro acetone biosynthesis driven by ATP regeneration

Published:2022 Issue:11 Volume:3 Page:1331-1341
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Kozaeva Ekaterina¹,Nieto-Domínguez Manuel¹^ORCID,Hernández Abril D.¹,Nikel Pablo I.¹^ORCID

Affiliation:

1. The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark

Abstract

The canonical acetone biosynthesis pathway provides an excellent starting point for C2-dependent synthetic metabolism. In this work, we established an ATP-efficient enzyme cascade for ketone biosynthesis from acetate with close-to-theoretical yields.

Funder

Novo Nordisk Fonden

Villum Fonden

H2020 Environment

Publisher

Royal Society of Chemistry (RSC)

Subject

Biochemistry, Genetics and Molecular Biology (miscellaneous),Molecular Biology,Biochemistry,Chemistry (miscellaneous)

Link

http://pubs.rsc.org/en/content/articlepdf/2022/CB/D2CB00170E

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1. High‐throughput colorimetric assays optimized for detection of ketones and aldehydes produced by microbial cell factories