A novel biocompatible europium ligand for sensitive time-gated immunodetection

Author:

Sayyadi Nima12345,Connally Russell E.674,Try Andrew534

Affiliation:

1. ARC Centre of Excellence for Nanoscale Biophotonics (CNBP)

2. Macquarie University

3. Sydney

4. Australia

5. Department of Chemistry and Biomolecular Sciences Macquarie University

6. Department of Physics and Astronomy

7. Macquarie University Sydney

Abstract

We describe the synthesis of a novel hydrophilic derivative of a tetradentate β-diketone europium ligand that was used to prepare an immunoconjugate probe against Giardia lamblia cysts. We used a Gated Autosynchronous Luminescence Detector (GALD) to obtain high quality delayed luminescence images of cells 30-fold faster than ever previously reported.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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