Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cells-Reference-Cited by-同舟云学术

Super RLuc8-sFv; a new luciferase-labeled probe for detection of human CD4+ cells

Published:2017 Issue:3 Volume:13 Page:470-475
ISSN:1742-206X
Container-title:Molecular BioSystems
language:en
Short-container-title:Mol. BioSyst.

Author:

Safavi A.¹²³⁴⁵,Emamzadeh R.¹²³⁴⁵^ORCID,Nazari M.⁶⁷⁸⁹⁵,Ehsani M.¹²³⁴⁵,Zarkesh-Esfahani S. H.¹²³⁴⁵,Rahgozar S.¹²³⁴⁵

Affiliation:

1. Department of Biology

2. Faculty of Sciences

3. University of Isfahan

4. Isfahan

5. Iran

6. Monoclonal Antibody Research Center

7. Avicenna Research Institute

8. ACECR

9. Tehran

Abstract

A probe has been designed, produced and assayed for the detection of T-helper cells. The sFv fragment fused to the C-terminus of super Renilla luciferase 8 (Super RLuc8) and the probe successfully used for detection of human CD4+ cells using luminometer.

Publisher

Royal Society of Chemistry (RSC)

Subject

Molecular Biology,Biotechnology

Link

http://pubs.rsc.org/en/content/articlepdf/2017/MB/C6MB00652C

Reference32 articles.

1. Crystal structure of a 3B3 variant-A broadly neutralizing HIV-1 scFv antibody

2. A. K. Abbas , A. H.Lichtman and S.Pillai, Cellular and molecular immunology: with STUDENT CONSULT Online Access, Elsevier Health Sciences, 2011

3. Panel on Antiretroviral Guidelines for Adults and Adolescents , Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents, Department of Health and Human Services, 2016, http://www.aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf, accessed 18 Jan 2017

4. In Vivo Binding and Retention of CD4-Specific DARPin 57.2 in Macaques

5. Engineering antibodies for clinical applications

Cited by 7 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Label-Free and Bioluminescence-Based Nano-Biosensor for ATP Detection;Biosensors;2022-10-24

2. Novel luminescent affiprobes for molecular detection of Staphylococcus aureus using flow cytometry;Journal of Applied Microbiology;2020-08-10

3. The occurrence and ecology of Renilla spp. over the southwest Atlantic continental shelf (28–34ºS);Regional Studies in Marine Science;2020-02

4. Detection of a prostate cancer cell line using a bioluminescent affiprobe: An attempt to develop a new molecular probe for ex vivo studies;International Journal of Biological Macromolecules;2019-10

5. A luminescent biosensor for ex vivo detection of HER2-positive breast cancer based on a novel affiprobe;Analytical Methods;2019