Computational design and clinical demonstration of a copper nanocluster based universal immunosensor for sensitive diagnostics

Author:

Kurdekar Aditya Dileep1234ORCID,Sai Manohar Chelli5234ORCID,Chunduri L. A. Avinash674ORCID,Haleyurgirisetty Mohan Kumar89101112,Hewlett Indira K.89101112,Kamisetti Venkataramaniah1234ORCID

Affiliation:

1. Department of Physics

2. Sri Sathya Sai Institute of Higher Learning

3. Prasanthinilayam 515134

4. India

5. Department of Chemistry

6. Andhra Pradesh MedTech Zone

7. Vishakhapatnam

8. Laboratory of Molecular Virology

9. Center for Biologics Evaluation and Research (CBER)

10. Food and Drug Administration

11. Silver Spring

12. USA

Abstract

Glutathione capped copper nanoclusters were bioconjugated to streptavidin protein using EDC-NHS coupling to engineer the Copper Nanocluster Immunosensor (CuNIS), which was tested for its efficiency by using HIV-1 p24 as the model analyte.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

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