A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC-Reference-Cited by-同舟云学术

A colourimetric high-throughput screening system for directed evolution of prodigiosin ligase PigC

Published:2020 Issue:61 Volume:56 Page:8631-8634
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Brands Stefanie¹²³⁴^ORCID,Brass Hannah U. C.⁵⁶⁷⁸⁴^ORCID,Klein Andreas S.⁵⁶⁷⁸⁴^ORCID,Pietruszka Jörg⁵⁶⁷⁸⁴^ORCID,Ruff Anna Joëlle¹²³⁴^ORCID,Schwaneberg Ulrich¹²³⁴⁹^ORCID

Affiliation:

1. Lehrstuhl für Biotechnologie

2. RWTH Aachen University

3. 52074 Aachen

4. Germany

5. Institute of Bioorganic Chemistry

6. Heinrich Heine University Düsseldorf located at Forschungszentrum Jülich

7. Stetternicher Forst

8. 52426 Jülich

9. DWI-Leibniz Institut für Interaktive Materialien

Abstract

A colourimetric high-throughput screening system was developed for the first directed evolution campaign on PigC towards production of artificial prodiginines.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2020/CC/D0CC02181D

Reference30 articles.

1. The biosynthesis and regulation of bacterial prodiginines

2. Chemistry and Biology of Roseophilin and the Prodigiosin Alkaloids: A Survey of the Last 2500 Years

3. Structure, Chemical Synthesis, and Biosynthesis of Prodiginine Natural Products

4. Characterization of a prodigiosin synthetase PigC from Serratia marcescens jx-1 and its application in prodigiosin analogue synthesis

5. Swiveling Domain Mechanism in Pyruvate Phosphate Dikinase,

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