Revisiting staining of biological samples for electron microscopy: perspectives for recent research-Reference-Cited by-同舟云学术

Revisiting staining of biological samples for electron microscopy: perspectives for recent research

Published:2021 Issue:3 Volume:8 Page:685-699
ISSN:2051-6347
Container-title:Materials Horizons
language:en
Short-container-title:Mater. Horiz.

Author:

Kuchenbrod Maren T.¹²³⁴²,Schubert Ulrich S.¹²³⁴²^ORCID,Heintzmann Rainer³⁴²⁵⁴,Hoeppener Stephanie¹²³⁴²^ORCID

Affiliation:

1. Laboratory of Organic and Macromolecular Chemistry (IOMC) Friedrich Schiller University Jena

2. Germany

3. Jena Center for Soft Matter (JCSM) Friedrich Schiller University Jena

4. 07743 Jena

5. Institute of Physical Chemistry Friedrich Schiller University Jena

Abstract

This review revisits staining protocols for electron microscopy focussing on the visualization of active sites, i.e. enzymes, metabolites or proteins, in cells and tissues, which were never established as standard protocols in electron microscopy.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrical and Electronic Engineering,Process Chemistry and Technology,Mechanics of Materials,General Materials Science

Link

http://pubs.rsc.org/en/content/articlepdf/2021/MH/D0MH01579B

Reference211 articles.

1. The ups and downs of beam damage, contrast and noise in biological electron microscopy

2. Transmission electron microscopy in molecular structural biology: A historical survey

3. Application of transmission electron microscopy to the clinical study of viral and bacterial infections: Present and future

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5. Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine

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