Online protein digestion in membranes between capillary electrophoresis and mass spectrometry-Reference-Cited by-同舟云学术

Online protein digestion in membranes between capillary electrophoresis and mass spectrometry

Published:2023 Issue:7 Volume:148 Page:1611-1619
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Ryan Kendall A.¹,Bruening Merlin L.¹²^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA

2. Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA

Abstract

This research employs pepsin-containing membranes to digest proteins online after a capillary electrophoresis separation and prior to tandem mass spectrometry, allowing the peptides from a protein to enter the mass spectrometer in a single plug.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN00106G

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