AIE-nanoparticle assisted ultra-deep three-photon microscopy in the in vivo mouse brain under 1300 nm excitation

Author:

Li Dongyu12345ORCID,Zhang Hequn678910,Streich Lina L.1112131415,Wang Yalun1617185,Lu Ping1920215ORCID,Wang Ling11121314,Prevedel Robert11121314ORCID,Qian Jun678910ORCID

Affiliation:

1. Britton Chance Center for Biomedical Photonics

2. Wuhan National Laboratory for Optoelectronics

3. Huazhong University of Science and Technology

4. Wuhan

5. China

6. State Key Laboratory of Modern Optical Instrumentations

7. Centre for Optical and Electromagnetic Research

8. College of Optical Science and Engineering

9. International Research Center for Advanced Photonics

10. Zhejiang University

11. Cell Biology and Biophysics Unit

12. European Molecular Biology Laboratory (EMBL)

13. Heidelberg 69126

14. Germany

15. Candidate for Joint PhD Degree from EMBL and Heidelberg University

16. Zhejiang Gongshang Univ

17. Sch Informat & Elect Engn

18. Hangzhou 310018

19. State Key Lab of Supramolecular Structure and Materials

20. Jilin University

21. Changchun 130012

Abstract

Here we present organic AIE nanoparticles that feature high absorption cross-section under NIR-IIa three-photon excitation, which enables ultra-deep three-photon fluorescence imaging in the in vivo mouse brain.

Funder

European Commission

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Natural Science Foundation of Zhejiang Province

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Materials Science

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