Developing a transwell millifluidic device for studying blood–brain barrier endothelium-Reference-Cited by-同舟云学术

Developing a transwell millifluidic device for studying blood–brain barrier endothelium

Published:2022 Issue:23 Volume:22 Page:4603-4620
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Harding Ian C.¹,O'Hare Nicholas R.²,Vigliotti Mark²,Caraballo Alex²,Lee Claire I.¹,Millican Karina¹,Herman Ira M.³⁴,Ebong Eno E.¹²⁵^ORCID

Affiliation:

1. Department of Bioengineering, Northeastern University, Boston, MA, USA

2. Department of Chemical Engineering, Northeastern University, 360 Huntington Avenue, 129 Interdisciplinary Science and Engineering Complex, Boston, MA, 02115, USA

3. Department of Developmental, Molecular, and Chemical Biology, Tufts School of Graduate Biomedical Sciences, Boston, MA, USA

4. Center for Innovations in Wound Healing Research, Tufts University School of Medicine, Boston, MA, USA

5. Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA

Abstract

To investigate blood–brain barrier function, a millifluidic device was developed for endothelium, astrocyte, and pericyte co-culture on transwell inserts, fluid flow incorporation, and immunocytochemical, western blot, and permeability assays.

Funder

National Science Foundation

Northeastern University

National Heart, Lung, and Blood Institute

American Heart Association

Office of the Vice Provost for Research, Tufts University

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2022/LC/D2LC00657J