A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells-Reference-Cited by-同舟云学术

A dual-fluorophore sensor approach for ratiometric fluorescence imaging of potassium in living cells

Published:2021 Issue:5 Volume:12 Page:1720-1729
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Wang Zeming¹²³⁴,Detomasi Tyler C.¹²³⁴^ORCID,Chang Christopher J.¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemistry

2. University of California

3. Berkeley

4. USA

5. Department of Molecular and Cell Biology

Abstract

We report a dual-fluorophore approach for ratiometric fluorescent imaging of K⁺ levels in live cells. Intracellular esterases cleave RPS-1 to detach the K⁺-responsive fluorophore (PS525) from its internal standard (Coumarin 343).

Funder

National Institutes of Health

Human Frontier Science Program

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/SC/D0SC03844J

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