Quantifying Siglec-sialylated ligand interactions: a versatile <sup>19</sup>F-T<sub>2</sub> CPMG filtered competitive NMR displacement assay-Reference-Cited by-同舟云学术

Quantifying Siglec-sialylated ligand interactions: a versatile ¹⁹F-T₂ CPMG filtered competitive NMR displacement assay

Published:2024 Issue:27 Volume:15 Page:10612-10624
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Atxabal Unai¹,Fernández Andrea¹²,Moure Maria Jesús¹,Sobczak Klaudia¹,Nycholat Corwin³,Almeida-Marrero Verónica⁴⁵,Oyenarte Iker¹,Paulson James C.³,Escosura Andrés de la⁴⁵^ORCID,Torres Tomás⁴⁵⁶^ORCID,Reichardt Niels C.²⁷^ORCID,Jiménez-Barbero Jesús¹⁸⁹¹⁰^ORCID,Ereño-Orbea June¹⁸

Affiliation:

1. Chemical Glycobiology Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain

2. Glycotechnology Laboratory, CIC biomaGUNE, Paseo Miramon 194, San Sebastian, 20014, Spain

3. Departments of Molecular Medicine and Immunology & Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA

4. Department of Organic Chemistry, Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, 28049 Madrid, Spain

5. Institute for Advanced Research in Chemical Sciences (IAdChem), Universidad Autónoma de Madrid, C/Francisco Tomás y Valiente 7, 28049 Madrid, Spain

6. Instituto Madrileño de Estudios Avanzados (IMDEA)-Nanociencia, C/Faraday 9, 28049 Madrid, Spain

7. CIBER-BBN, Paseo Miramon 194, San Sebastian, 20014, Spain

8. Ikerbasque, Basque Foundation for Science, Bilbao, Spain

9. Department of Organic & Inorganic Chemistry, Faculty of Science and Technology, University of the Basque Country, EHU-UPV, 48940 Leioa, Bizkaia, Spain

10. Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias, 28029 Madrid, Spain

Abstract

Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are integral cell surface proteins crucial for the regulation of immune responses and the maintenance of immune tolerance through interactions with sialic acids.

Funder

HORIZON EUROPE Marie Sklodowska-Curie Actions

Agencia Estatal de Investigación

European Commission

H2020 European Research Council

Comunidad de Madrid

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC01723D

Reference70 articles.

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4. Siglecs that Associate with DAP12

5. Crystal Structure of the N-Terminal Domain of Sialoadhesin in Complex with 3′ Sialyllactose at 1.85 Å Resolution