Spatial distribution and temporal evolution of DRONPA-fused SNAP25 clusters in adrenal chromaffin cells

Author:

Antoku Yasuko1234,Dedecker Peter5678,Pinheiro Paulo S.1234,Vosch Tom92104,Sørensen Jakob Balslev1234

Affiliation:

1. Department of Neuroscience and Pharmacology and Center for Biomembranes in Nanomedicine (CBN)

2. University of Copenhagen

3. 2200 Copenhagen

4. Denmark

5. Department of Chemistry and Institute for Nanoscale Physics and Chemistry

6. Katholieke Universiteit Leuven

7. 3001 Heverlee

8. Belgium

9. Nano-Science Center/Department of Chemistry

10. 2100 Copenhagen

Abstract

Sub-diffraction imaging of DRONPA-fused SNAP-25 in adrenal chromaffin cells allows tracking protein cluster dynamics over relative long time (20 min) and reveal that clusters have rich dynamics going from staying constant to disappearing and reappearing within minutes.

Publisher

Springer Science and Business Media LLC

Subject

Physical and Theoretical Chemistry

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