Gold labelling of a green fluorescent protein (GFP)-tag inside cells using recombinant nanobodies conjugated to 2.4 nm thiolate-coated gold nanoparticles-Reference-Cited by-同舟云学术

Gold labelling of a green fluorescent protein (GFP)-tag inside cells using recombinant nanobodies conjugated to 2.4 nm thiolate-coated gold nanoparticles

Published:2021 Issue:24 Volume:3 Page:6940-6948
ISSN:2516-0230
Container-title:Nanoscale Advances
language:en
Short-container-title:Nanoscale Adv.

Author:

Groysbeck Nadja¹^ORCID,Donzeau Mariel¹^ORCID,Stoessel Audrey¹,Haeberle Anne-Marie²,Ory Stéphane²^ORCID,Spehner Danièle³,Schultz Patrick³^ORCID,Ersen Ovidiu⁴^ORCID,Bahri Mounib⁴^ORCID,Ihiawakrim Dris⁴^ORCID,Zuber Guy¹^ORCID

Affiliation:

1. Université de Strasbourg – CNRS, UMR 7242 Laboratoire de Biotechnologie et Signalisation Cellulaire, Boulevard Sébastien Brant, 67400 Illkirch, France

2. Centre National de la Recherche Scientifique, Université de Strasbourg, Institut des Neurosciences Cellulaires et Intégratives, F-67000 Strasbourg, France

3. Université de Strasbourg – Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67400 Illkirch, France

4. Université de Strasbourg – CNRS, UMR 7504, Institut de Physique et Chimie des Matériaux de Strasbourg (IPCMS), 23 rue de Loess, 67034 Strasbourg, France

Abstract

A specific and non-covalent approach is reported to associate the targeting ability of nanobodies with the detectability of 2.4 nm gold nanoparticles for immunolabelling of cellular effectors using light and high-resolution electron microscopy.

Funder

Agence Nationale de la Recherche

Ligue Contre le Cancer

Centre National de la Recherche Scientifique

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/NA/D1NA00256B

Reference48 articles.

1. Super-resolution microscopy demystified