Cloning and expression of the flavin reductase LuxG fromPhotobacterium leiognathiYL and its improvement for NADH detection-Reference-Cited by-同舟云学术

Cloning and expression of the flavin reductase LuxG fromPhotobacterium leiognathiYL and its improvement for NADH detection

Published:2020 Issue:2 Volume:19 Page:274-280
ISSN:1474-905X
Container-title:Photochemical & Photobiological Sciences
language:en
Short-container-title:Photochem. Photobiol. Sci.

Author:

Xuan Guanhua¹²³⁴⁵,Xiao Qilin¹²³⁴⁵,Wang Jingxue¹²³⁴⁵^ORCID,Lin Hong¹²³⁴⁵

Affiliation:

1. Food Safety Laboratory

2. College of Food Science and Engineering

3. Ocean University of China

4. Qingdao 266003

5. China

Abstract

LuxG fromP. leiognathiYL was successfully purified and characterized. Based on this, a coupled pure enzyme bioluminescent system was established and used for NADH detection, which showed higher sensitivity than existing bioluminescent systems.

Funder

National Basic Research Program of China

Publisher

Springer Science and Business Media LLC

Subject

Physical and Theoretical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/PP/C9PP00435A

Reference28 articles.

1. The effect of Hg2+on the bioluminescence ofPhotobacterium leiognathi

2. J. X. Wang , L. L.Zhu , C. X.Mei , Y. Q.Wang and H.Lin , Proceedings of 2009 International Conference of Natural Product and Traditional Medicine , 2009 , vol. 1 and 2 , pp. 167–171

3. Expression of genes encoding the luciferase fromPhotobacterium leiognathiinEscherichia coliRosetta (DE3) and its application in NADH detection

4. Molecular Mechanisms of Bacterial Bioluminescence

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