Development of a protocol of isolation of nanoparticles from patients' broncho-alveolar lavages for their in vitro toxicity assessment

Author:

Bernal Kévin1,Kose Ozge1,Leclerc Lara1,Vergnon Jean-Michel23,Pourchez Jérémie1ORCID,Forest Valérie1ORCID

Affiliation:

1. Mines Saint-Etienne, Univ Jean Monnet, INSERM, U 1059 Sainbiose, Centre CIS, CS 62362 42023, 158 cours Fauriel, F-42023 Saint-Etienne Cedex 2, France

2. Univ Lyon, Univ Jean Monnet, INSERM, U 1059 Sainbiose, F-42023 Saint-Etienne, France

3. CHU Saint-Etienne, Service de Pneumologie, F-42055 Saint-Etienne, France

Abstract

Objective: assessment of the toxicity of particles contained in broncho-alveolar lavages (BAL) from patients. Issue: BAL are conditioned in sodium hypochlorites solution ⇒ cannot be directly incubated with cells. Solution: 2 proposed approaches.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

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