Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways
Author:
Affiliation:
1. State Key Laboratory of Bioreactor Engineering
2. New World Institute of Biotechnology
3. East China University of Science and Technology
4. Shanghai 200237
5. China
6. Ocean Univ China
7. Coll Food Sci & Engn
8. Qingdao 266003
Abstract
Based on this mechanism, a nitrilase was engineered to shift the reaction pathway from formation of acid to formation of amide.
Funder
Natural Science Foundation of Shanghai
National Natural Science Foundation of China
Publisher
Royal Society of Chemistry (RSC)
Subject
Catalysis
Link
http://pubs.rsc.org/en/content/articlepdf/2017/CY/C7CY00060J
Reference44 articles.
1. Metal-catalyzed amide bond forming reactions in an environmentally friendly aqueous medium: nitrile hydrations and beyond
2. CeO2-catalyzed nitrile hydration to amide: reaction mechanism and active sites
3. Key green chemistry research areas—a perspective from pharmaceutical manufacturers
4. Efficient and practical transition metal-free catalytic hydration of organonitriles to amides
5. Arene–Ruthenium(II) Complexes Containing Inexpensive Tris(dimethylamino)phosphine: Highly Efficient Catalysts for the Selective Hydration of Nitriles into Amides
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