The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells-Reference-Cited by-同舟云学术

The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells

Published:2014 Issue:13 Volume:139 Page:3296-3304
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Schrems Angelika¹²³,Phillips John⁴⁵⁶⁷,Casey Duncan⁴⁵⁶⁷,Wylie Douglas⁴⁵⁶⁷,Novakova Mira⁴⁵⁶⁷,Sleytr Uwe B.¹²³,Klug David⁴⁵⁶⁷,Neil Mark A. A.⁴⁵⁶⁷,Schuster Bernhard¹²³,Ces Oscar⁴⁵⁶⁷

Affiliation:

1. Department of Nanobiotechnology

2. University of Natural Resources and Life Sciences

3. Vienna, 1190 Austria

4. The Proxomics Group

5. Institute of Chemical Biology

6. Imperial College London

7. London, SW7 2AZ UK

Abstract

Samples of cell membrane were non-destructively removed from individual, live cells using optically trapped beads, and deposited into a supported lipid bilayer mounted on an S-layer protein-coated substrate.

Funder

Austrian Academy of Sciences

Engineering and Physical Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AN/C4AN00059E

Reference57 articles.

1. Stochastic protein expression in individual cells at the single molecule level

2. Dynamic Proteomics of Individual Cancer Cells in Response to a Drug