Development of a mix-and-read assay for human asprosin using antibody–oligonucleotide probes and thermofluorimetric analysis-Reference-Cited by-同舟云学术

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Development of a mix-and-read assay for human asprosin using antibody–oligonucleotide probes and thermofluorimetric analysis

Published:2024 Issue:35 Volume:16 Page:6057-6063
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Hu Juan¹,Easley Christopher J.¹^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry, Auburn University, Auburn, AL, USA

Abstract

A custom thermofluorimetric analysis (TFA) method was developed for asprosin, a recently discovered adipokine hormone. The assay workflow was accomplished in less than 1 hour, and it was validated with human serum samples.

Funder

National Institutes of Health

National Institute of Diabetes and Digestive and Kidney Diseases

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AY/D3AY01175E

Reference43 articles.

1. Microfluidics: reframing biological enquiry

2. Profiling Glucose-Stimulated and M3 Receptor-Activated Insulin Secretion Dynamics from Islets of Langerhans Using an Extended-Lifetime Fluorescence Dye

3. Active Flow Control and Dynamic Analysis in Droplet Microfluidics

4. Luminescent oxygen channeling assay (LOCI): sensitive, broadly applicable homogeneous immunoassay method

5. Nucleic-Acid Driven Cooperative Bioassays Using Probe Proximity or Split-Probe Techniques

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