Engineered unnatural ubiquitin for optimal detection of deubiquitinating enzymes-Reference-Cited by-同舟云学术

Engineered unnatural ubiquitin for optimal detection of deubiquitinating enzymes

Published:2020 Issue:23 Volume:11 Page:6058-6069
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Rut Wioletta¹²³⁴^ORCID,Zmudzinski Mikolaj¹²³⁴^ORCID,Snipas Scott J.⁵⁶⁷,Bekes Miklos⁸⁹¹⁰⁷,Huang Tony T.⁸⁹¹⁰⁷,Drag Marcin¹²³⁴⁵^ORCID

Affiliation:

1. Department of Chemical Biology and Bioimaging

2. Wroclaw University of Science and Technology

3. 50-370 Wroclaw

4. Poland

5. Sanford Burnham Prebys Medical Discovery Institute

6. La Jolla

7. USA

8. Department of Biochemistry & Molecular Pharmacology

9. New York University School of Medicine

10. New York

Abstract

Herein we present a workflow for design and synthesis of novel selective Ub-based tools for DUBs. Selectivity is achieved by incorporation of unnatural amino acids into the Ub C-terminal epitope.

Funder

Fundacja na rzecz Nauki Polskiej

Narodowe Centrum Nauki

European Regional Development Fund; European Union

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2020/SC/D0SC01347A

Reference47 articles.

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5. The Papain-Like Protease from the Severe Acute Respiratory Syndrome Coronavirus Is a Deubiquitinating Enzyme

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