Exploiting dCas9 fusion proteins for dynamic assembly of synthetic metabolons-Reference-Cited by-同舟云学术

Exploiting dCas9 fusion proteins for dynamic assembly of synthetic metabolons

Published:2019 Issue:57 Volume:55 Page:8219-8222
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Berckman Emily A.¹²³⁴⁵,Chen Wilfred⁵³⁴^ORCID

Affiliation:

1. Department of Chemistry and Biochemistry

2. University of Delaware

3. Newark

4. USA

5. Department of Chemical and Biomolecular Engineering, University of Delaware

Abstract

We report a new approach for conditional cellulosome formation using toehold-gated dCas9-guided protein assembly. Binding of SpdCas9-CBD is activated by a RNA trigger to unblock the spacer region of thgRNA by toehold-mediated strand displacement.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2019/CC/C9CC04002A

Reference26 articles.

1. Escherichia coli as a host for metabolic engineering

2. Microbial engineering for the production of advanced biofuels

3. Biomolecular scaffolds for enhanced signaling and catalytic efficiency

4. Metabolon formation and metabolic channeling in the biosynthesis of plant natural products

5. The surprising dynamics of scaffolding proteins

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