Light-inducible activation of cell cycle progression in Xenopus egg extracts under microfluidic confinement

Author:

Bisht Jitender12345,LeValley Paige62345,Noren Benjamin62345,McBride Ralph6234ORCID,Kharkar Prathamesh7894,Kloxin April7894ORCID,Gatlin Jesse12345,Oakey John62345ORCID

Affiliation:

1. Department of Molecular Biology

2. University of Wyoming

3. Laramie

4. USA

5. Cell Organization and Division Group

6. Department of Chemical Engineering

7. Department of Chemical and Biomolecular Engineering

8. University of Delaware

9. Newark

Abstract

Protein release from microfluidically-confined photodegradable hydrogels allows dynamic cycling of cell-free Xenopus egg extracts, a widely used and biochemically tractable model system.

Funder

Marine Biological Laboratory

National Institute of General Medical Sciences

National Science Foundation

Burroughs Wellcome Fund

University of Delaware Research Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

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