Design and synthesis of fluorescence-labeled nucleotide with a cleavable azo linker for DNA sequencing

Author:

Tan Lianjiang12345,Liu Yazhi12345,Yang Qinglai12345,Li Xiaowei67345,Wu Xin-Yan8910115,Gong Bing121314515,Shen Yu-Mei12345,Shao Zhifeng67345

Affiliation:

1. Shanghai Center for Systems Biomedicine

2. Key Laboratory of Systems Biomedicine

3. Shanghai Jiao Tong University

4. Shanghai 200240

5. China

6. Bio-ID Center

7. School of Bio-medical Engineering

8. Key Laboratory for Advanced Materials and Institute of Fine Chemicals

9. School of Chemistry & Molecular Engineering

10. East China University of Science and Technology

11. Shanghai 200237

12. College of Chemistry

13. Beijing Normal University

14. Beijing 100875

15. Department of Chemistry

Abstract

A 3′-OH unblocked nucleotide analogue with cleavable azo linker was synthesized as a potential reversible terminator for DNA sequencing.

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

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