Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis-Reference-Cited by-同舟云学术

Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis

Published:2018 Issue:32 Volume:9 Page:6692-6702
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Espinoza-Cara Andrés¹²³⁴⁵^ORCID,Zitare Ulises⁶⁷⁸⁹¹⁰^ORCID,Alvarez-Paggi Damián⁶⁷⁸⁹¹⁰^ORCID,Klinke Sebastián¹¹¹²¹⁰³¹³^ORCID,Otero Lisandro H.¹¹¹²¹⁰³¹³^ORCID,Murgida Daniel H.⁶⁷⁸⁹¹⁰^ORCID,Vila Alejandro J.¹²³⁴⁵^ORCID

Affiliation:

1. Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR)

2. Rosario

3. Argentina

4. Área Biofísica

5. Departamento de Química Biológica

6. Departamento de Química Inorgánica

7. Analítica y Química Física—INQUIMAE

8. Facultad de Ciencias Exactas y Naturales

9. Universidad de Buenos Aires—CONICET

10. Buenos Aires

11. Fundación Instituto Leloir

12. IIBBA-CONICET

13. Plataforma Argentina de Biología Estructural y Metabolómica PLABEM.

Abstract

Loop directed mutagenesis leads to a cupredoxin withthe strongest copper–thiolate bond known to date, high reduction potential and imidazole binding properties.

Funder

Agencia Nacional de Promoción Científica y Tecnológica

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/SC/C8SC01444B

Reference84 articles.

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2. Structural and Functional Aspects of Metal Sites in Biology

3. Copper Active Sites in Biology