Synthesis and immunological evaluation of TLR1/2 ligand-conjugated RBDs as self-adjuvanting vaccine candidates against SARS-CoV-2

Author:

Manabe Yoshiyuki12ORCID,Gárate-Reyes Brandon1ORCID,Ito Keita1,Hurtado-Guerrero Ramón3456ORCID,Kabayama Kazuya12,Fukase Koichi127ORCID

Affiliation:

1. Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

2. Forefront Research Center, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan

3. Institute of Biocomputation and Physics of Complex Systems (BIFI), University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I + D, Zaragoza, Spain

4. Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark

5. Laboratorio de Microscopías Avanzada (LMA), University of Zaragoza, Mariano Esquillor s/n, Campus Rio Ebro, Edificio I + D, Zaragoza, Spain

6. Fundación ARAID, Zaragoza, Spain

7. Center for Advanced Modalities and DDS, Osaka University, 11 Yamadaoka, Suita, Osaka, 565-0871, Japan

Abstract

We synthesized and evaluated Pam3CSK4-conjugated receptor binding domain (RBD)/deglycosylated RBD as potential anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine candidates.

Funder

Japan Agency for Medical Research and Development

Fusion Oriented REsearch for disruptive Science and Technology

Japan Society for the Promotion of Science

Core Research for Evolutional Science and Technology

Ministerio de Ciencia e Innovación

Publisher

Royal Society of Chemistry (RSC)

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