Fluorescence detection of apolipoprotein E gene polymorphisms based on oligonucleotide ligation and magnetic separation

Author:

Wen Wan-Chen1,Lin Yi-Hui2,Duh Tsai-Hui3ORCID,Chen Chun-Hsien4,Feng Chia-Hsien1567ORCID,Chen Yen-Ling1489

Affiliation:

1. Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

2. School of Pharmacy, College of Pharmacy, China Medical University, Taichung 406040, Taiwan

3. Department of Medicinal and Applied Chemistry, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

4. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan

5. College of Professional Studies, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan

6. Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan

7. Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan

8. Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi 621301, Taiwan

9. Center for Nano Bio-Detection, National Chung Cheng University, Chia-Yi 621301, Taiwan

Abstract

A fluorescence detection system coupled with oligonucleotide ligation and magnetic separation was developed to identify two single-nucleotide polymorphisms for the APOE gene.

Funder

Ministry of Science and Technology, Taiwan

China Medical University, Taiwan

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Reference35 articles.

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