Standard addition method for rapid, cultivation-independent quantification of <i>Legionella pneumophila</i> cells by qPCR in biotrickling filters-Reference-Cited by-同舟云学术

Standard addition method for rapid, cultivation-independent quantification of Legionella pneumophila cells by qPCR in biotrickling filters

Published:2024 Issue:10 Volume:149 Page:2978-2987
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Schwaiger Gerhard¹,Matt Marco¹,Streich Philipp¹^ORCID,Bromann Sarah²,Clauß Marcus²,Elsner Martin¹^ORCID,Seidel Michael¹^ORCID

Affiliation:

1. Institute of Water Chemistry, Chair of Analytical Chemistry and Water Chemistry, Technical University of Munich, Lichtenbergstraße 4, 85748 Garching, Germany

2. Thuenen-Institute for Agricultural Technology, Bundesallee 47, D-38116 Braunschweig, Germany

Abstract

A standard addition method for qPCR by spiking the target bacteria increases the trueness of the qPCR results in complex matrices.

Funder

Bundesministerium für Ernährung und Landwirtschaft

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D3AN02207B

Reference45 articles.

1. A Comparison of Legionella pneumophila Occurrence in Hot Water Tanks and Instantaneous Devices in Domestic, Nosocomial, and Community Environments

2. Sporadic Legionnaires' disease: the role of domestic electric hot-water tanks

3. Legionella species colonization in cooling towers: Risk factors and assessment of control measures

4. Presence of Legionella spp. in cooling towers: the role of microbial diversity, Pseudomonas, and continuous chlorine application

5. Legionella risk in evaporative cooling systems and underlying causes of associated breaches in health and safety compliance

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