Label-free offline versus online activity methods for nucleoside diphosphate kinase b using high performance liquid chromatography-Reference-Cited by-同舟云学术

Label-free offline versus online activity methods for nucleoside diphosphate kinase b using high performance liquid chromatography

Published:2016 Issue:15 Volume:141 Page:4733-4741
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Lima Juliana Maria¹²³⁴⁵,Salmazo Vieira Plínio⁶⁷⁸⁹,Cavalcante de Oliveira Arthur Henrique¹²³⁴⁵,Cardoso Carmen Lúcia¹²³⁴⁵

Affiliation:

1. Departamento de Química

2. Grupo de Cromatografia de Bioafinidade e Produtos Naturais

3. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto

4. Universidade de São Paulo

5. 14040-901

6. Laboratório Nacional de Biociências (LNBio)

7. Centro Nacional de Pesquisa em Energia e Materiais (CNPEM)

8. Campinas

9. Brazil

Abstract

Label-free methodologies for nucleoside diphosphate kinase fromLeishmaniaspp. (LmNDKb): anofflineLC-UV assay for solubleLmNDKb and anonlinetwo-dimensional LC-UV system based on immobilizedLmNDKb to help screenLmNDKb ligands and measure NDKb activity.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2016/AN/C6AN00655H

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4. Characterization of membrane-associated Pseudomonas aeruginosa Ras-like protein Pra, a GTP-binding protein that forms complexes with truncated nucleoside diphosphate kinase and pyruvate kinase to modulate GTP synthesis

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