Conformational fingerprinting with Raman spectroscopy reveals protein structure as a translational biomarker of muscle pathology

Author:

Alix James J. P.123ORCID,Plesia Maria1,Dudgeon Alexander P.45ORCID,Kendall Catherine A.4,Hewamadduma Channa36,Hadjivassiliou Marios36,Gorman Gráinne S.789,Taylor Robert W.78,McDermott Christopher J.123,Shaw Pamela J.123,Mead Richard J.12,Day John C.10

Affiliation:

1. Sheffield Institute for Translational Neuroscience, University of Sheffield, UK

2. Neuroscience Institute, University of Sheffield, Western Bank, Sheffield, UK

3. National Institute for Health and Care Research Sheffield Biomedical Research Centre, Sheffield, UK

4. Biophotonics Research Unit, Gloucestershire Hospitals NHS Foundation Trust, UK

5. Department of Physics and Astronomy, University of Exeter, UK

6. Department of Neurology, Academic Directorate of Neurosciences, Sheffield Teaching Hospitals NHS Foundation Trust, Royal Hallamshire Hospital, UK

7. Wellcome Centre for Mitochondrial Research, Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK

8. NHS Highly Specialised Service for Rare Mitochondrial Disorders, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK

9. National Institute for Health and Care Research Newcastle Biomedical Research Centre, Newcastle upon Tyne, UK

10. Interface Analysis Centre, School of Physics, University of Bristol, UK

Abstract

Using a combination of peak fitting and matrix factorisation techniques we show that neuromuscular disease in both human biopsies and preclinical models manifests alterations in protein secondary structure. Created with biorender.com.

Funder

Academy of Medical Sciences

Medical Research Council

National Institute for Health Research

Wellcome Trust Centre for Mitochondrial Research

Wellcome Trust

Publisher

Royal Society of Chemistry (RSC)

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