Comparison and optimization of nanoscale extracellular vesicle imaging by scanning electron microscopy for accurate size-based profiling and morphological analysis

Author:

Cavallaro Sara12345ORCID,Hååg Petra6785ORCID,Viktorsson Kristina6785ORCID,Krozer Anatol91011125,Fogel Kristina91011125,Lewensohn Rolf678513ORCID,Linnros Jan12345,Dev Apurba12345ORCID

Affiliation:

1. Department of Applied Physics

2. School of Engineering Sciences

3. KTH Royal Institute of Technology

4. 10691 Stockholm

5. Sweden

6. Department of Oncology-Pathology

7. Karolinska Institutet

8. 17164 Solna

9. Department of Smart Hardware

10. Division of Digital Systems

11. Research Institutes of Sweden AB

12. 40014 Gothenburg

13. Theme Cancer, Medical Unit Head and Neck, Lung, and Skin Tumors

Abstract

Nanoscale extracellular vesicles (EVs) have emerged as a valuable source of disease biomarkers. Here, we present a direct visual approach for their accurate morphological and size-based profiling by using scanning electron microscopy.

Funder

Stockholms Läns Landsting

Familjen Erling-Perssons Stiftelse

Vetenskapsrådet

Cancerfonden

Karolinska Institutet

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

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