On-line coupling of continuous-flow gel electrophoresis with inductively coupled plasma-mass spectrometry to quantitatively evaluate intracellular metal binding properties of metallochaperones HpHypA and HpHspA in E. coli cells

Author:

Wang Yuchuan123,Hu Ligang123,Yang Xinming123,Chang Yuen-Yan123,Hu Xuqiao123,Li Hongyan123,Sun Hongzhe123

Affiliation:

1. Department of Chemistry

2. The University of Hong Kong

3. Hong Kong, P. R. China

Abstract

Quantitative analysis of metal selectivity of overexpressed metalloproteins in cells by GE-ICP-MS.

Funder

Research Grants Council, University Grants Committee, Hong Kong

Publisher

Oxford University Press (OUP)

Subject

Metals and Alloys,Biochemistry,Biomaterials,Biophysics,Chemistry (miscellaneous)

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