Microfluidic bead encapsulation above 20 kHz with triggered drop formation
Author:
Affiliation:
1. Department of Bioengineering and Therapeutic Sciences
2. University of California, San Francisco
3. San Francisco
4. USA
5. California Institute for Quantitative Biosciences (QB3)
6. Chan Zuckerberg Biohub
Abstract
Bead-triggered breakup of a fluid jet into monodispersed droplets improves the throughput of bead-based droplet workflows, enabling the analysis of large populations and the detection of rare events.
Funder
amfAR, The Foundation for AIDS Research
National Institute of Allergy and Infectious Diseases
Publisher
Royal Society of Chemistry (RSC)
Subject
Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering
Link
http://pubs.rsc.org/en/content/articlepdf/2018/LC/C8LC00514A
Reference19 articles.
1. Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
2. Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations
3. Microbead display by in vitro compartmentalisation: selection for binding using flow cytometry
4. Single-Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions
5. Droplet Barcoding for Single-Cell Transcriptomics Applied to Embryonic Stem Cells
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