Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry-Reference-Cited by-同舟云学术

Establishing ion ratio thresholds based on absolute peak area for absolute protein quantification using protein cleavage isotope dilution mass spectrometry

Published:2014 Issue:21 Volume:139 Page:5439-5450
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Loziuk Philip L.¹²³⁴,Sederoff Ronald R.⁵⁶³⁴,Chiang Vincent L.⁵⁶³⁴,Muddiman David C.¹²³⁴

Affiliation:

1. W. M. Keck Fourier Transform Mass Spectrometry Laboratory

2. Department of Chemistry

3. North Carolina State University

4. Raleigh, USA

5. Forest Biotechnology Group

6. Department of Forestry and Environmental Resources

Abstract

Relative abundance values and their associated variability are dynamic and dependent on absolute abundance.

Funder

National Science Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2014/AN/C4AN00567H

Reference23 articles.

1. Absolute Quantification of C-Reactive Protein in Human Plasma Derived from Patients with Epithelial Ovarian Cancer Utilizing Protein Cleavage Isotope Dilution Mass Spectrometry

2. Multi-peptide nLC-PC-IDMS-SRM-based Assay for the quantification of biomarkers in the chicken ovarian cancer model

3. Absolute Quantification of the Model Biomarker Prostate-Specific Antigen in Serum by LC−MS/MS Using Protein Cleavage and Isotope Dilution Mass Spectrometry

4. Absolute quantification of free glutathione and cysteine in aquatic insects using isotope dilution and selected reaction monitoring

5. Application of Selected Reaction Monitoring for Multiplex Quantification of Clinically Validated Biomarkers in Formalin-Fixed, Paraffin-Embedded Tumor Tissue

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