Rational engineering and synthetic applications of a high specificity BiFC probe derived from Springgreen-M-Reference-Cited by-同舟云学术

Rational engineering and synthetic applications of a high specificity BiFC probe derived from Springgreen-M

Published:2022 Issue:19 Volume:147 Page:4326-4336
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Sun Yuao¹,Wang Yao¹,Chen Keyang²,Sun Yujie¹³,Wang Sheng⁴⁵^ORCID

Affiliation:

1. State Key Laboratory of Membrane Biology, Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing 100871, China

2. Yuanpei College, Peking University, Beijing 100871, China

3. National Biomedical Imaging Center, School of Future Technology, Peking University, Beijing 100871, China

4. School of Biopharmacy, China Pharmaceutical University, Nanjing 211198, China

5. Changzhou High-Tech Research Institute of Nanjing University, Changzhou 213164, China

Abstract

A high signal-to-noise (S/N) ratio BiFC assay was developed for efficient detection and flexible visualization of protein–protein interactions under physiological conditions in live cells.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Jiangsu Province

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/AN/D2AN01124G

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