Generic phosphatase activity detection using zinc mediated aggregation modulation of polypeptide-modified gold nanoparticles
Author:
Affiliation:
1. Division of Molecular Physics
2. Department of Physics
3. Chemistry and Biology
4. Linköping University
5. SE-583 36 Linköping, Sweden
Abstract
A generic plasmonic assay for rapid detection and characterization of a wide range of phosphatases using different natural substrates is described that exploit the enzymatic release of inorganic phosphate to control the aggregation of nanoparticles functionalized with a designed Zn2+responsive polypeptide.
Funder
Swedish Foundation for Strategic Research
Publisher
Royal Society of Chemistry (RSC)
Subject
General Materials Science
Link
http://pubs.rsc.org/en/content/articlepdf/2014/NR/C4NR02791D
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