Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate-Reference-Cited by-同舟云学术

Bisulfite-free and base-resolution analysis of 5-methylcytidine and 5-hydroxymethylcytidine in RNA with peroxotungstate

Published:2019 Issue:16 Volume:55 Page:2328-2331
ISSN:1359-7345
Container-title:Chemical Communications
language:en
Short-container-title:Chem. Commun.

Author:

Yuan Fang¹²³⁴⁵,Bi Ying¹²³⁴,Siejka-Zielinska Paulina¹²³⁴,Zhou Ying-Lin⁵⁶⁷⁸⁹^ORCID,Zhang Xin-Xiang⁵⁶⁷⁸⁹,Song Chun-Xiao¹²³⁴^ORCID

Affiliation:

1. Ludwig Institute for Cancer Research and Target Discovery Institute

2. Nuffield Department of Medicine

3. University of Oxford

4. UK

5. Beijing National Laboratory for Molecular Sciences (BNLMS)

6. MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering

7. College of Chemistry

8. Peking University

9. Beijing 100871

Abstract

WO-Seq: a bisulfite-free and base-resolution sequencing method based on peroxotungstate oxidation is presented for the identification of hm⁵C sites in the transcriptome. Combining the peroxotungstate oxidation with TET enzyme oxidation, m⁵C can also be detected in a procedure termed TET-Assisted WO-Seq (TAWO-Seq).

Funder

Cancer Research UK

Ludwig Institute for Cancer Research

China Scholarship Council

National Institute on Handicapped Research

Conrad N. Hilton Foundation

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,Metals and Alloys,Surfaces, Coatings and Films,General Chemistry,Ceramics and Composites,Electronic, Optical and Magnetic Materials,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2019/CC/C9CC00274J

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