Development of a selective and highly sensitive fluorescence assay for nucleoside triphosphate diphosphohydrolase1 (NTPDase1, CD39)

Author:

Lee Sang-Yong12345ORCID,Luo Xihuan12345,Namasivayam Vigneshwaran12345ORCID,Geiss Jennifer12345,Mirza Salahuddin12345,Pelletier Julie678,Stephan Holger9101112ORCID,Sévigny Jean6781314ORCID,Müller Christa E.12345ORCID

Affiliation:

1. PharmaCenter Bonn

2. Pharmaceutical Institute

3. Pharmaceutical Chemistry I

4. University of Bonn

5. D-53121 Bonn

6. Centre de Recherche du CHU de Québec – Université Laval

7. Québec City

8. Canada

9. Institute of Radiopharmaceutical Cancer Research

10. Helmholtz-Zentrum Dresden - Rossendorf

11. 01328 Dresden

12. Germany

13. Départment de Microbiologie-Infectiologie et d'Immunologie

14. Faculté de Médecine

Abstract

A new fluorescence-based assay is useful for monitoring CD39 reactions and enables low picomolar detection of nucleotides.

Funder

Canadian Institutes of Health Research

Fonds de Recherche du Québec - Santé

Natural Sciences and Engineering Research Council of Canada

Deutsche Forschungsgemeinschaft

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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