Live-cell fluorescence imaging: assessment of thioflavin T uptake in human epidermal carcinoma cells
Author:
Affiliation:
1. ICCE Institute
2. Molecular Imaging Center
3. Mallinckrodt Institute of Radiology
4. Washington University School of Medicine
5. St. Louis
6. Students and Teachers as Research Scientists (STARS) Program
7. USA
Abstract
Live-cell imaging shows that thioflavin-T is recognized by Pgp as its transport substrate due to its either exclusion- or LY335979-induced accumulation in MDR cells.
Funder
National Heart, Lung, and Blood Institute
National Institute on Aging
Publisher
Royal Society of Chemistry (RSC)
Subject
Pharmaceutical Science,Biochemistry,Drug Discovery,Molecular Medicine,Pharmacology,Organic Chemistry
Link
http://pubs.rsc.org/en/content/articlepdf/2018/MD/C8MD00101D
Reference30 articles.
1. Analyzing Thioflavin T Binding to Amyloid Fibrils by an Equilibrium Microdialysis-Based Technique
2. Simultaneous Measurement of Amyloid Fibril Formation by Dynamic Light Scattering and Fluorescence Reveals Complex Aggregation Kinetics
3. Thioflavin T as an amyloid dye: fibril quantification, optimal concentration and effect on aggregation
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