Rapid measurement of hemoglobin-oxygen dissociation by leveraging Bohr effect and Soret band bathochromic shift

Author:

Sekyonda Zoe1ORCID,An Ran2ORCID,Goreke Utku2ORCID,Man Yuncheng2ORCID,Monchamp Karamoja23,Bode Allison23,Zhang Qiaochu2,El-Gammal Yasmin4,Kityo Cissy5ORCID,Kalfa Theodosia A.46ORCID,Akkus Ozan127ORCID,Gurkan Umut A.1278ORCID

Affiliation:

1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH, USA

2. Department of Mechanical and Aerospace Engineering, Case Western Reserve University, 10900 Euclid Ave., Glennan Building 616B, Cleveland, OH, 44106, USA

3. Division of Hematology and Oncology, University Hospitals Cleveland Medical Center, Cleveland, OH, USA

4. Division of Hematology, Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA

5. The Joint Clinical Research Center, Kampala, Uganda

6. Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA

7. Department of Orthopedics, Case Western Reserve University, Cleveland, OH, USA

8. Case Comprehensive Cancer Center, Case Western Reserve University, Cleveland, OH, USA

Abstract

We present a rapid hemoglobin-oxygen dissociation measurement approach to screen for and detect hemoglobin-oxygen affinity abnormalities and to evaluate the function and efficacy of hemoglobin modifying therapies within minutes.

Funder

National Institute of Diabetes and Digestive and Kidney Diseases

American Society of Hematology

National Heart, Lung, and Blood Institute

National Institute of Biomedical Imaging and Bioengineering

Publisher

Royal Society of Chemistry (RSC)

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