e-MagnetoMethyl IP: a magnetic nanoparticle-mediated immunoprecipitation and electrochemical detection method for global DNA methylation-Reference-Cited by-同舟云学术

e-MagnetoMethyl IP: a magnetic nanoparticle-mediated immunoprecipitation and electrochemical detection method for global DNA methylation

Published:2021 Issue:11 Volume:146 Page:3654-3665
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Farhana Fatema Zerin¹²³⁴,Umer Muhammad⁵⁶⁷,Saeed Ayad⁵⁶⁷^ORCID,Pannu Amandeep Singh⁸⁹¹⁰⁷¹¹^ORCID,Husaini Sediqa¹²⁶⁷,Sonar Prashant⁸⁹¹⁰⁷¹¹^ORCID,Firoz Shakhawat H.¹²³⁴^ORCID,Shiddiky Muhammad J. A.⁵⁶⁷¹²⁶^ORCID

Affiliation:

1. Department of Chemistry

2. Bangladesh University of Engineering and Technology (BUET)

3. Dhaka 1000

4. Bangladesh

5. Queensland Micro- and Nanotechnology Centre (QMNC)

6. Griffith University

7. Australia

8. School of Chemistry and Physics

9. Queensland University of Technology (QUT)

10. Brisbane 4000

11. Centre for Material Science

12. School of Environment and Science (ESC)

Abstract

e-MagnetoMethyl IP is a new method for electrochemical analysis of global DNA methylation. It avoids bisulfite treatment, PCR amplification, and enzyme-based signal generation and can detect differences as low as 5% in global DNA methylation levels.

Funder

Australian Research Council

Bangladesh University of Engineering and Technology

Queensland University of Technology

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AN/D1AN00345C

Reference51 articles.

1. Principles and challenges of genome-wide DNA methylation analysis