Improving the activity of endoglucanase I (EGI) from Saccharomyces cerevisiae by DNA shuffling

Author:

Wang Xu12345ORCID,Rong Liang6789,Wang Mingfu1234,Pan Yingjie1234,Zhao Yong1234,Tao Fang5104

Affiliation:

1. College of Food Science and Technology

2. Shanghai Ocean University

3. Shanghai

4. China

5. School of Life Sciences

6. USC School of Pharmacy

7. University of Southern California

8. Los Angeles

9. USA

10. Anhui Agricultural University

Abstract

To enhance the endo-β-1,4-glucanase activity of three mixedTrichodermasp. (Trichoderma reesei, Trichoderma longibrachiatum, andTrichoderma pseudokoningii), we optimized the efficiency of the encoding gene using DNA shuffling andSaccharomyces cerevisiaeINVSc1 as a host.

Funder

Science and Technology Commission of Shanghai Municipality

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

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