Improving the activity of endoglucanase I (EGI) from Saccharomyces cerevisiae by DNA shuffling-Reference-Cited by-同舟云学术

Improving the activity of endoglucanase I (EGI) from Saccharomyces cerevisiae by DNA shuffling

Published:2017 Issue:73 Volume:7 Page:46246-46256
ISSN:2046-2069
Container-title:RSC Adv.
language:en
Short-container-title:RSC Adv.

Author:

Wang Xu¹²³⁴⁵^ORCID,Rong Liang⁶⁷⁸⁹,Wang Mingfu¹²³⁴,Pan Yingjie¹²³⁴,Zhao Yong¹²³⁴,Tao Fang⁵¹⁰⁴

Affiliation:

1. College of Food Science and Technology

2. Shanghai Ocean University

3. Shanghai

4. China

5. School of Life Sciences

6. USC School of Pharmacy

7. University of Southern California

8. Los Angeles

9. USA

10. Anhui Agricultural University

Abstract

To enhance the endo-β-1,4-glucanase activity of three mixedTrichodermasp. (Trichoderma reesei, Trichoderma longibrachiatum, andTrichoderma pseudokoningii), we optimized the efficiency of the encoding gene using DNA shuffling andSaccharomyces cerevisiaeINVSc1 as a host.

Funder

Science and Technology Commission of Shanghai Municipality

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/RA/C6RA26508A

Reference62 articles.

1. CELLULOSE DEGRADATION IN ANAEROBIC ENVIRONMENTS

2. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives

3. Fungal cellulase systems. Comparison of the specificities of the cellobiohydrolases isolated from Penicillium pinophilum and Trichoderma reesei