A microscopy-compatible temperature regulation system for single-cell phenotype analysis – demonstrated by thermoresponse mapping of microalgae-Reference-Cited by-同舟云学术

A microscopy-compatible temperature regulation system for single-cell phenotype analysis – demonstrated by thermoresponse mapping of microalgae

Published:2021 Issue:9 Volume:21 Page:1694-1705
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Andersson Martin¹²³⁴⁵^ORCID,Johansson Sofia¹²³⁴⁵^ORCID,Bergman Henrik¹²³⁴⁵^ORCID,Xiao Linhong⁶²³⁷⁵^ORCID,Behrendt Lars⁶²³⁷⁵^ORCID,Tenje Maria¹²³⁴⁵^ORCID

Affiliation:

1. Dept. Materials Science and Engineering

2. Science for Life Laboratory

3. Uppsala University

4. 751 03 Uppsala

5. Sweden

6. Dept. Organismal Biology

7. 752 36 Uppsala

Abstract

A heat-stage for spatiotemporal temperature control in microfluidic chips is developed. The technology is used for mapping thermoresponses of single cells of Symbiodinium in order to improve our understanding of temperature-induced coral bleaching.

Funder

Vetenskapsrådet

Knut och Alice Wallenbergs Stiftelse

H2020 European Research Council

Uppsala Universitet

Science for Life Laboratory

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2021/LC/D0LC01288B